Epub Oct Cold atmospheric-pressure plasma CAP is a relatively new method used for bacterial inactivation. CAP is ionized gas that can be generated by applying an electric current to air or a feeding gas.
Abstract Biofilms are communities of microorganism enclosed in an extracellular matrix attached to various surfaces. Biofilms and motilities may represent negative impacts in health and industrial sectors, however they also play crucial ecological role. The main objective of this study was to estimate biofilm formation and motilities abilities of three common bacteria under different culture condition media of growth and pH.
Results show that pH variation from 5 to 8 and composition of media growth minimum and complex media have little influence on biofilm formation by Staphylococcus aureus ATCC and Escherichia coli ATCC However, biofilm formation by Pseudomonas aeruginosa ATCC is inhibited at pH 5 and 6 in both complex and minimum media.
This preliminary study demonstrated that pH may influence bacterial behavior formation particularly for P.
Biofilms are surface-associated communities enclosed within an extracellular matrix mainly composed of polysaccharides, proteins, nucleic acids, lipids and other macromolecules and chemicals  which protect bacteria cells against environmental assaults and predation, including antimicrobials and host defenses.
Indeed, comprehension of these behaviors is important to elaborate adequate strategy in order to struggle against bacterial infection or to exploit their biotechnological potential. Biofilm Formation and Quantification The formation of biofilm was studied on well micro-plates and quantified by a crystal violet staining protocol as previously described .
Motility Assays Swimming, swarming and twitching motilities were examined by using LB agar at different pH 5, 6, 7 and 8 0. Bacteria spreading from the inoculation spot were measured with sliding caliper after adequate incubation time.
The inoculation spot is generally around 6mm and spreading less than 8mm was considered as non-motile mechanism. Statistics All experiments were performed in triplicate and repeated in 3 independent assays.
Indeed, there is no significant difference between biofilm formed in pH5, 6, 7 and 8 Figure 1. In regard of motilities, S. A Biofilm formation by S. B Swimming motility of S. C Swarming motility of S. D Twitching motility of S. All experiments were performed in triplicate with three independent assays.
Biofilm formation by Escherichia coli is similar to S. However, they develop more consistent biofilm when grown in minimum media. In regard of motilities, Escherichia coli was unable to spread in all BB medium agar and unable to swim in complex medium Figure 2.
However, a slight swarming 10mm which is not influenced by pH variation is observed in complex medium and interestingly, Escherichia coli twitch more under acidic pH than neutral and alkaline pH condition.
A Biofilm formation by E. B Swimming motility of E. C Swarming motility of E. D Twitching motility of E.Jan 08, · Pseudomonas aeruginosa, a motile, non-fermenting Gram-negative bacterium, is an opportunistic pathogen implicated in respiratory infections, urinary tract infections, gastrointestinal infections, keratitis, otitis media, and bacteremia in patients with .
A mutant of Pseudomonas aeruginosa PAO1, which overproduces alginates, was obtained by screening transposon mutagenesis libraries. A mucoid P. aeruginosa strain (A) was used for .
Pseudomonas aeruginosa is the key bacterial agent of cystic fibrosis (CF) lung infections, and the most important pathogen in progressive and severe CF lung srmvision.com opportunistic pathogen can grow and proliferate in patients, and exposure can occur in hospitals and other healthcare settings.
Jul 21, · The opportunistic pathogen Pseudomonas aeruginosa causes severe infections, particularly in immunocompromised individuals and patients with cystic fibrosis (CF). The progressive deterioration of the lungs caused by chronic P. aeruginosa infections and, thus, persistent inflammation are currently the main causes of morbidity and mortality in patients with CF ().
pleiotropic effects, which include modulation of the Pseudomonas aeruginosa.5 These encouraging results prompted a trial of atorvastatin treatment in a group of patients with more severe bronchiectasis who were and chemoattractants by cytometric bead array (BD Biosciences) and.
Pseudomonas aeruginosa is an opportunistic pathogen that infects burns, wounds, surgical incisions and sites of catheterization.
It is the most common cause of infections of .